A major question in developmental biology is understanding how cellular fate decisions are regulated precisely in space and time.

Fortunately, we can now begin to observe these processes with modern imaging approaches, which allow the tracking of cell fate decision events and cellular rearrangements by live 3D time-lapse microscopy.

This development is shifting our understanding of pattern formation in development away from static models towards models based on the principles of dynamical systems and statistical mechanics.

In this episode of Listen In, hear how light-sheet microscopy is the technique of choice for volumetric live imaging of 3D samples at high speed with minimal phototoxicity.

See two user examples of how light-sheet microscopy is helping scientists understand the biological process of intestinal and brain organoid development.

We also explore the technology behind the Leica Viventis LS2 Live, which combines dual-view illumination and detection in an open-top configuration.

You will also learn how this unique configuration allows easy sample mounting and long-term imaging from small embryos and organoids to large multicellular systems.

Watch the full presentation here: https://microscopyfocus.com/multi-view-light-sheet-microscopy-to-track-cell-lineages-in-tissue-morphogenesis/

"Browse all episodes of the Listen In Series here: https://listen-in.bitesizebio.com/

In this episode, learn how AI-powered segmentation, spatial analysis, and phenotyping can help you gain new insights for 3D images with complex morphological measurements featuring up to 15 biomarkers.

See how to characterize tissue microenvironments and examine the differences between normal and disease tissues without the need to code or train Deep Learning models—all with Aivia.

Plus, get your 3D spatial biology results faster with an inbuilt, enhanced Deep Learning model that can accurately detect and partition cells with morphological variations.

You will discover how to leverage your expertise or simple automation to classify cells into different phenotypes and interactively explore them in their spatial context.

We also demonstrate how, with a few clicks, you can produce key measurements such as percentage distribution, Pearson correlation coefficient, and dimensionality reduction. You will also learn how to visualize the relationship between biomarkers and clusters using dendrograms.

Watch the full presentation here: https://microscopyfocus.com/enhancing-3d-spatial-biology-with-ai-simplified-insights-for-all/

Browse all episodes of the Listen In Series here: https://listen-in.bitesizebio.com/

Imaging scientists have a critical educational role in improving rigor and reproducibility by sharing their technical expertise and providing intellectual contributions in all aspects of image-based science.

They participate in creating, developing, and disseminating guidelines, resources, and tools to improve image-based research.

In this episode, learn what impacts reproducibility in microscopy, discover resources and initiatives for improving education, rigor, and reproducibility, and see how collaboration drives innovation.

Watch the full presentation here: https://microscopyfocus.com/accelerate-scientific-progress-the-power-of-reproducibility-collaboration-and-new-imaging-technologies/

Browse all episodes of the Listen In Series here: https://listen-in.bitesizebio.com/

Immunoassays play an indispensable role in scientific research. They facilitate the detection and quantification of specific analytes, enabling in-depth investigations into disease mechanisms, biomarker discovery, and evaluation of the potential of therapeutic interventions.

Sample selection and preparation are of utmost importance, as they have a direct impact on the accuracy, reliability, and integrity of the obtained results.

Properly selecting and preparing samples ensures that they are representative of the population under study and that the analytes of interest are preserved in their native state.

In addition, controlling and mitigating matrix interference is crucial in immunoassays to minimize the influence of sample components that may hinder the accuracy of measurements.

In this episode, hear from immunoassay experts as they guide you through all these areas.

Watch the full presentation here: https://events.bitesizebio.com/inside-immunoassays-best-practices/join

Browse all episodes of the Listen In Series here: https://listen-in.bitesizebio.com/

The brain is an immune-privileged organ, meaning it is protected from the entry of pathogens, blood circulating factors, and immune cells by a physical barrier called the blood-brain barrier (BBB).

But pathological events such as ischemia (stroke), trauma (traumatic brain injury), or tumor growth (gliomas and brain metastasis), activate the vascular endothelium, disturb or disrupt the BBB and allow immune cell homing.

In this episode, discover how innate immunity can sustain deleterious effects following brain trauma, and the technological developments enabling longitudinal studies into post-trauma lesion remodeling.

Watch the full presentation here: https://microscopyfocus.com/windows-on-neurovascular-pathologies/

Browse all episodes of the Listen In Series here: https://listen-in.bitesizebio.com/

Tissue heterogeneity is a significant challenge when analyzing proteins in tissue samples to identify disease states.

In this episode, explore how combining laser microdissection with mass spectrometry (LCM-MS) on fixed and stained tissues lets you explore the protein networks within pathological features to shed light on their molecular functions.

Using hallmark lesions of lung fibrosis in the honeycomb airways and fibroblastic foci as an example, you'll hear how this technology can detect over 4,900 proteins from tissue volumes as low as 0.005 mm3.

Watch the full presentation here: https://events.bitesizebio.com/unleashing-fibrotic-secrets/join

Browse all episodes of the Listen In Series here: https://listen-in.bitesizebio.com/

Join Advanced Workflow Specialists for a virtual reality showcase of Leica's confocal microscopy platform, STELLARIS.

In this episode of Listen In, discover how to enhance your confocal imaging from performing multicolor experiments with clear, sharp images to capturing fluorescence intensity and lifetime-based information simultaneously.

Watch the full presentation here: https://microscopyfocus.com/confocal-microscopy-stellaris-showcase/

Browse all episodes of the Listen In Series here: https://listen-in.bitesizebio.com/

The fundamental cellular mechanisms of internalization and trafficking are crucial to many areas of cell biology, especially the proper function of therapeutic antibodies.

Antibodies intended for use as antibody-drug conjugates (ADCs) should specifically bind to target cells and rapidly internalize via endocytosis.
However, the ability to study these processes has historically been limited by the lack of tools that directly monitor the internalization and subsequent catabolism of extracellular material.

In this episode, get a demo of rapid, high-throughput methods to screen for antibody internalization. You will also hear about techniques to directly visualize the internalization of therapeutic antibodies and ADCs in live cells for structure-activity relationship studies.

You will also learn how the insights provided by these approaches have the potential to accelerate biotherapeutic lead generation and development.

Watch the full presentation here: https://events.bitesizebio.com/tools-techniques-for-the-study-of/join

Browse all episodes of the Listen In Series here: https://listen-in.bitesizebio.com/